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Amaxa sf cell line 4d-nucleofector x kit l
Sf Cell Line 4d Nucleofector X Kit L, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+nucleofector+kit+l/sf+cell+line+nucleofector+4d+kit/pmc12094058-40-1-0
Average 90 stars, based on 1 article reviews
sf cell line 4d-nucleofector x kit l - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

In Vitro:

Article Title: Antisense nucleic acid that induces skipping of exon 50
Article Snippet: .. In Vitro Test of Exon 50 Skipping in Human Dystrophin Gene (1) Testing Method Using an Amaxa Cell Line Nucleofector Kit L and Nucleofector II (Lonza), 0.1 to 1 μM of each antisense oligomer of Table 1 was transfected to 3.5×105 of RD cells (human rhabdomyosarcoma cell line, CCL-136, purchased from ATCC)). ..

Transfection:

Article Title: Antisense nucleic acid that induces skipping of exon 50
Article Snippet: .. In Vitro Test of Exon 50 Skipping in Human Dystrophin Gene (1) Testing Method Using an Amaxa Cell Line Nucleofector Kit L and Nucleofector II (Lonza), 0.1 to 1 μM of each antisense oligomer of Table 1 was transfected to 3.5×105 of RD cells (human rhabdomyosarcoma cell line, CCL-136, purchased from ATCC)). ..

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: Fibroblasts from a DMD patient with deletion of exon 45 of theDMD gene (GM05112) were obtained from the Coriell Institute for Medical Research. .. After transfection of a retroviral vector for coexpressing humanMYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described.25 Transfection of PMOs into cells PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. ..

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: After transfection of a retroviral vector for coexpressing human MYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described. .. PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. .. Total RNA was extracted from RD cells or cells from a DMD patient, and RT-PCR was performed with 400 ng (RD cells) or 50 ng (DMD cells) of total RNA using a forward primer (5′-GCTCAGGTCGGATTGACATT-3′) and a reverse primer (5′-GGGCAACTCTTCCACCAGTA-3′) designed to be complementary to exons 43 and 47 as previously described.

Article Title: RORgamma modulators and uses thereof
Article Snippet: Transfection of EL4 cells was achieved with Amaxa electroporation apparatus (Amaxa Biosystems, Germany), as per the manufacturer's protocols, for the EL4 cells (Amaxa Cell Line Nucleofector Kit L, Amaxa Biosystems). .. Transfection of EL4 cells was achieved with Amaxa electroporation apparatus (Amaxa Biosystems, Germany), as per the manufacturer's protocols, for the EL4 cells (Amaxa Cell Line Nucleofector Kit L, Amaxa Biosystems). ..

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [ ]. .. 2 × 10 6 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [ ], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 10 5 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma.
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [67]. .. 2 × 106 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [70], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 105 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Retroviral:

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: Fibroblasts from a DMD patient with deletion of exon 45 of theDMD gene (GM05112) were obtained from the Coriell Institute for Medical Research. .. After transfection of a retroviral vector for coexpressing humanMYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described.25 Transfection of PMOs into cells PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. ..

Plasmid Preparation:

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: Fibroblasts from a DMD patient with deletion of exon 45 of theDMD gene (GM05112) were obtained from the Coriell Institute for Medical Research. .. After transfection of a retroviral vector for coexpressing humanMYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described.25 Transfection of PMOs into cells PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. ..

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [ ]. .. 2 × 10 6 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [ ], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 10 5 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma.
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [67]. .. 2 × 106 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [70], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 105 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Fluorescence:

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: Fibroblasts from a DMD patient with deletion of exon 45 of theDMD gene (GM05112) were obtained from the Coriell Institute for Medical Research. .. After transfection of a retroviral vector for coexpressing humanMYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described.25 Transfection of PMOs into cells PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. ..

FACS:

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: Fibroblasts from a DMD patient with deletion of exon 45 of theDMD gene (GM05112) were obtained from the Coriell Institute for Medical Research. .. After transfection of a retroviral vector for coexpressing humanMYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described.25 Transfection of PMOs into cells PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. ..

Electroporation:

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: Fibroblasts from a DMD patient with deletion of exon 45 of theDMD gene (GM05112) were obtained from the Coriell Institute for Medical Research. .. After transfection of a retroviral vector for coexpressing humanMYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described.25 Transfection of PMOs into cells PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. ..

Article Title: Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide
Article Snippet: After transfection of a retroviral vector for coexpressing human MYOD and ZsGreen1, ZsGreen1-positive cells were sorted by fluorescence-activated cell sorting and induced to differentiate into myotubes as previously described. .. PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent. .. Total RNA was extracted from RD cells or cells from a DMD patient, and RT-PCR was performed with 400 ng (RD cells) or 50 ng (DMD cells) of total RNA using a forward primer (5′-GCTCAGGTCGGATTGACATT-3′) and a reverse primer (5′-GGGCAACTCTTCCACCAGTA-3′) designed to be complementary to exons 43 and 47 as previously described.

Article Title: RORgamma modulators and uses thereof
Article Snippet: Transfection of EL4 cells was achieved with Amaxa electroporation apparatus (Amaxa Biosystems, Germany), as per the manufacturer's protocols, for the EL4 cells (Amaxa Cell Line Nucleofector Kit L, Amaxa Biosystems). .. Transfection of EL4 cells was achieved with Amaxa electroporation apparatus (Amaxa Biosystems, Germany), as per the manufacturer's protocols, for the EL4 cells (Amaxa Cell Line Nucleofector Kit L, Amaxa Biosystems). ..

Bioprocessing:

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [ ]. .. 2 × 10 6 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [ ], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 10 5 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma.
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [67]. .. 2 × 106 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [70], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 105 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Control:

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [ ]. .. 2 × 10 6 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [ ], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 10 5 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Article Title: AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma.
Article Snippet: Subsequent to insult, a cellular ROS assay was performed using CellROX green (Thermo Fisher, Waltham, MA, USA) and flow cytometry as previously described [67]. .. 2 × 106 pLC cells were transfected (Amaxa biosystems, Nucleofector II/2B) with the Cell Line Nucleofector Kit L, using a 4.5:1 ratio of supplement to Nucleofector solution, and program C-005 with 2μg of AAV-eNdi1 plasmid DNA [70], or pAAV-MCS (Agilent Technologies Inc, Santa Clara, USA; control) as per manufacturer’s instructions. (Lonza Group AG, Basel, Switzerland). .. Following transfection, 1.5 × 105 LC cells were seeded onto 8-well imaging slides (Milenyi Biotec, Bergisch Gladback, Germany).

Knockdown:

Article Title: Synthetic oleanane triterpenoids suppress MYB oncogene activity and sensitize T-cell acute lymphoblastic leukemia cells to chemotherapy
Article Snippet: .. MYB siRNA-mediated knockdown was done with the Cell Line Nucleofector Kit L on the Amaxa Nucleofector II device (Cologne, Germany) using 2x10 6 MOLT-4 cells according to the instructions provided by the manufacturer. .. Cells were electroporated with 1 μM Silencer Select MYB (s9109, s9110) or negative control siRNAs (Thermo Fisher Scientific).

other:

Article Title: Comparison of two methods of sperm- and testis-mediated gene transfer in production of transgenic animals: A systematic review.
Article Snippet: DNErbB2 Electroporation – Syvyk and Syvyk (2019) SD rat pSEB- siMDR1 300 kHz- ultrasound irradiation – He et al. (2015) Mouse ICR pLV- EF1- a- Luc- CMVEGFP Polyethylene glycol (PEG) EGFP expression in seminiferous tubules Kim, Choi, et al. (2010) and Kim, Lee, et al. (2010) BALB/c mice pEGFP- N1 DMSO DMA Lipofectin Offspring 55.5% 27% 80% Amaral et al. (2011) Mouse ICR pEGFP- N1 ExGen500 Offspring 12.5% Ju et al. (2011) Mice (FVB/J) pCX- EGFP pK14- IRES2- EGFP pEGFP- SMAR1 Non- surgical in vivo injection and electroporation of mice testis Offspring 57%–62% Usmani et al. (2013) C57BL/6J pEGFPC1, pDsRed2- N1 Injection to rete testis/ electroporation – Michaelis et al. (2014) Mice pcDNA- appA- MxA (AMP) ExGen500 Offspring (8.8%) Bai et al. (2014) ICR mice pUC18- MMTV- TVAMP- 1 N- [1- (2,3- dioleoyloxy) propyl]- N,N,Ntrimethylammonium methylsulfate (DOTAP) liposomal transfection reagent Offspring 39.69% Chen et al. (2014) Mouse pWPXLd LVV/SSC SSC 72% Abbasi et al. (2015) BALB/c pX330- mcherry (CRISPR plasmids) Amaxa Cell Line Nucleofector Kit L/SSC Offspring 50% Wu et al. (2015) Mice FVB/NJ pCX- Egfp, Bucsn2- IRES2- Egfp Tris–HCl/plasmid Offspring 46% Usmani et al. (2016) ICR mice pAAV- CAG- mCherry or pAAV- CAG- Cre AAV1- AAV9/ polyethyleneimine MAX Offspring 58% Watanabe et al. (2017) FVB/N pAcGFP- N1 Bacterial magnetic particlespolyethylenimine- DNA complexes Offspring 73.8% Wang, Sun, et al. (2017) Mouse pDNA Gonadotropin hormonereleasing hormone (chitosan) – Boonthum et al. (2018) DBA, BDF1 CSII- Eif1a- IRES2Venus, CSIIEif1a- mNG (SV), pX330- U6- ChimericBB- CBh- hSpCas9 (LV) Incubation/virus packaging Natural mating 43% Shinohara and KanatsuShinohara (2020) Pig AAV2- CMV- EGFP, AAV2- PGK2- EGFP, AAV2- ACR3- EGFP, VSV- G.HIV.PGK1.



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